Tryptone is widely used in microbiology as a nutrient source for growing bacteria. It provides essential amino acids and peptides, making it a key component in culture media for the cultivation of non-fastidious microorganisms. In molecular biology, tryptone is often included in Luria-Bertani (LB) broth, a common medium for E. coli growth during plasmid preparation and protein expression studies. It also serves as a nitrogen source in fermentation processes for producing enzymes, antibiotics, and other biotechnological products, including vaccines. Additionally, tryptone is utilized in diagnostic applications, such as in the preparation of selective media for identifying specific bacterial strains. Its consistent composition and high nutrient content make it a reliable ingredient in various laboratory and industrial applications.