Used in agricultural practices to enhance soil fertility by promoting natural nitrogen fixation in leguminous and non-leguminous plants. These bacteria, when cultured in A Sugai’s medium, are optimized for high viability and inoculant efficiency, making them ideal for seed treatment and soil inoculation. They reduce the need for synthetic nitrogen fertilizers, supporting sustainable farming and organic crop production. Commonly applied in rice, wheat, and vegetable cultivation to improve plant growth and yield. Also utilized in reforestation and land restoration projects to enrich nutrient-poor soils.