Used in plant physiology research to quantify magnesium-protoporphyrin I (Mg-ProtoI), a key intermediate in the chlorophyll biosynthesis pathway. The ELISA kit enables sensitive and specific detection of Mg-ProtoI levels in plant tissue extracts, allowing researchers to study disruptions or enhancements in the tetrapyrrole synthesis pathway. It is particularly useful in analyzing mutants or transgenic plants with altered chlorophyll production, investigating photodynamic stress responses, and understanding regulatory mechanisms of photosynthesis-related metabolism. The assay supports high-throughput screening and provides quantitative data without requiring complex instrumentation like HPLC, making it accessible for routine lab use in plant biochemistry and molecular biology studies.