Binucleine 2 is primarily used in biological research as a selective inhibitor of the Polo-like kinase 4 (PLK4) enzyme. This application is crucial for studying cell division processes, particularly centriole duplication, which is essential for proper cell cycle regulation. By inhibiting PLK4, researchers can investigate the mechanisms underlying centrosome amplification and its implications in diseases such as cancer. Additionally, Binucleine 2 serves as a valuable tool in developmental biology to explore the role of PLK4 in tissue formation and organ development. Its specificity makes it a key compound for understanding cellular dynamics and potential therapeutic targets in PLK4-related disorders.