CFI-400437 is primarily utilized in research settings as a potent and selective inhibitor of Polo-like kinase 4 (PLK4), an enzyme involved in cell division and centriole duplication. Its application is significant in studying the mechanisms of cancer cell proliferation, as PLK4 overexpression is often associated with tumorigenesis. By inhibiting PLK4, CFI-400437 helps researchers explore its role in disrupting centrosome amplification, which is a common feature in many cancers. This chemical is also used in preclinical studies to evaluate its potential as a therapeutic agent for treating cancers that are dependent on PLK4 activity. Additionally, it aids in understanding the broader implications of PLK4 inhibition in cellular processes and its effects on tumor growth and survival.