Spermine dihydrate is widely used in biochemical research due to its role in stabilizing nucleic acids, particularly DNA. It is often employed in molecular biology techniques such as PCR (Polymerase Chain Reaction) to enhance the efficiency and specificity of DNA amplification. Additionally, it is utilized in cell culture studies to promote cell growth and viability, as it interacts with cellular membranes and intracellular components. In medical research, spermine dihydrate is investigated for its potential therapeutic applications, including its role in modulating cell proliferation and apoptosis in cancer studies. It also serves as a critical component in the study of polyamine metabolism and its implications in various physiological and pathological processes.