NS1643 is primarily used in research settings to study its effects on ion channels, particularly hERG potassium channels. It acts as an activator of these channels, which are crucial for cardiac repolarization. By modulating hERG channels, NS1643 helps researchers understand the mechanisms underlying cardiac arrhythmias and potential therapeutic interventions. Additionally, it is employed in studies exploring its potential to improve cardiac function in conditions like long QT syndrome. Beyond cardiac research, NS1643 is also investigated for its role in cancer biology, as hERG channels are implicated in tumor progression and cell proliferation. Its application in these areas contributes to the development of novel treatments for both cardiovascular diseases and cancer.