Bis-Tris precast gels are widely used in protein electrophoresis for separating proteins based on their molecular weight. The 8-20% gradient allows for the resolution of a broad range of protein sizes, making it suitable for analyzing complex protein mixtures. These gels are particularly useful in applications such as Western blotting, where precise protein separation is critical for accurate detection and analysis. The 1.5mm thickness ensures sufficient protein loading capacity while maintaining sharp band resolution. The glass-backed format provides durability and ease of handling during gel casting, running, and staining processes. These gels are commonly employed in research laboratories for studying protein expression, post-translational modifications, and protein-protein interactions. The 10-well configuration allows for multiple samples to be run simultaneously, increasing throughput and efficiency in experiments. Bis-Tris chemistry provides a stable pH environment, reducing protein modifications and improving the quality of separation. These gels are compatible with various staining methods, including Coomassie, silver stain, and fluorescent dyes, offering flexibility in detection. They are also suitable for downstream applications such as mass spectrometry, where high-resolution protein separation is essential for accurate identification. Overall, Bis-Tris precast gels are a valuable tool in proteomics research, providing reliable and reproducible results for protein analysis.