Tricine precast gel is widely used in protein electrophoresis, particularly for the separation of low molecular weight proteins and peptides. Its 16.5% concentration and 1.5mm thickness make it ideal for resolving small proteins in the range of 1-100 kDa with high resolution. The gel is commonly applied in biochemistry and molecular biology research for analyzing protein samples, such as in SDS-PAGE experiments. Its precast format ensures convenience and consistency, reducing preparation time and variability. The glass-backed design provides durability and ease of handling during staining and imaging processes. This gel is especially useful for studying complex protein mixtures, peptide mapping, and quality control in protein purification workflows.