Used for high-resolution separation of proteins in the molecular weight range of approximately 10–100 kDa. Ideal for SDS-PAGE applications where fine resolution is required, such as analyzing complex protein mixtures, verifying protein purity, or confirming protein subunit composition. The 8% acrylamide concentration provides optimal pore size for resolving medium-sized proteins with sharp, well-defined bands. Commonly used in western blotting, protein expression analysis, and quality control in molecular biology and biochemistry research. The Tris-Glycine buffer system ensures consistent migration and compatibility with downstream detection methods.