6β-Hydroxylevonorgestrel is a key active metabolite of levonorgestrel, primarily utilized in pharmacological research for hormonal contraception and hormone replacement therapies. It contributes to progestogenic activity by binding to progesterone receptors, supporting ovulation suppression and endometrial modification to prevent pregnancy. It plays a role in menstrual cycle regulation and is studied for its involvement in steroid hormone metabolic pathways. In research settings, it aids in evaluating the efficacy, safety, and duration of action of levonorgestrel-based contraceptive formulations, including bioanalytical methods for monitoring hormone levels in biological fluids like saliva or urine.