S-5751 is primarily utilized in biochemical research as a selective antagonist for the EP1 subtype of prostaglandin E2 receptors. Its application is significant in studies focused on understanding the role of prostaglandin E2 in various physiological and pathological processes, such as inflammation, pain, and fever. Researchers employ S-5751 to dissect the signaling pathways and mechanisms mediated by EP1 receptors, aiding in the development of targeted therapies for conditions like chronic pain and inflammatory diseases. Additionally, it is used in pharmacological experiments to evaluate the efficacy and specificity of new compounds designed to modulate prostaglandin activity.