ARD-61 is primarily utilized in research and development within the field of biochemistry and pharmacology. It serves as a valuable tool in studying enzyme mechanisms, particularly those involving proteases, due to its ability to selectively inhibit certain enzymatic activities. This compound is often employed in laboratory settings to investigate the role of specific enzymes in biological pathways, aiding in the understanding of disease mechanisms. Additionally, ARD-61 has potential applications in drug discovery, where it is used to screen and validate potential therapeutic targets. Its precise inhibitory properties make it a critical component in the development of enzyme-targeted therapies, contributing to advancements in treating conditions related to enzyme dysregulation.