BoNT-IN-1 is primarily used in research settings to study the mechanisms of botulinum neurotoxin (BoNT) and its effects on neural signaling. It serves as an inhibitor, helping scientists understand how BoNT disrupts neurotransmitter release, which is crucial for developing therapeutic strategies against botulism. Additionally, BoNT-IN-1 is explored for its potential in treating conditions related to excessive neurotransmitter release, such as certain neuromuscular disorders. Its application in pharmacological studies aids in identifying new drug targets and improving treatments for diseases involving synaptic dysfunction.