This chemical is widely used in biochemical research as a substrate for caspase-3 and related caspases, which are enzymes involved in apoptosis (programmed cell death). It helps in studying and quantifying caspase activity in vitro, providing insights into cellular mechanisms of apoptosis. Researchers use it to assess the effectiveness of drugs or compounds that modulate apoptotic pathways, making it valuable in cancer research and drug development. Additionally, it is employed in assays to understand diseases linked to dysregulated cell death, such as neurodegenerative disorders. Its colorimetric properties allow for easy detection and measurement of enzymatic activity.