Suc-Leu-Tyr-AMC (Suc-LY-AMC) is widely used as a fluorogenic substrate in biochemical research, particularly for studying protease activity. It is specifically designed to measure the activity of enzymes like chymotrypsin, trypsin, and other serine or cysteine proteases. When cleaved by these enzymes, the AMC (7-amino-4-methylcoumarin) group is released, producing a fluorescent signal that can be quantified. This property makes it a valuable tool in enzyme kinetics studies, high-throughput screening for drug discovery, and investigating protease-related biological pathways. Its application is also extended to cell-based assays and diagnostic research to monitor protease activity in various disease models.