Used as a fluorogenic substrate in caspase-9 enzyme activity assays. The compound enables detection of proteolytic cleavage through the release of a fluorescent group (AFC), allowing real-time monitoring of caspase activity in cell lysates or biochemical assays. Commonly applied in studies of programmed cell death pathways, particularly the intrinsic apoptotic pathway in research related to cancer and neurodegenerative diseases. Its selectivity for caspase-9 makes it valuable for distinguishing this initiator caspase from other caspases in complex biological samples. Widely used in screening compounds that modulate apoptosis.