Used as a fluorogenic substrate in enzymatic assays to measure the activity of proteases, particularly trypsin-like serine proteases. The compound releases a fluorescent signal upon cleavage at the arginine residue, allowing real-time monitoring of enzyme kinetics. Commonly applied in high-throughput screening for drug discovery, especially in studies involving coagulation factors, inflammatory enzymes, and cancer-related proteolytic pathways. Its high specificity and sensitivity make it suitable for both purified enzyme systems and complex biological samples.