Dansyl-DL-methionine cyclohexylammonium salt serves as a fluorescent substrate in biochemical and enzymatic assays, particularly for studying proteases and aminopeptidases involved in methionine metabolism, such as methionine aminopeptidase. The dansyl group enables sensitive detection of enzymatic activity through fluorescence changes upon peptide bond cleavage or modification. It is widely used in high-throughput screening for enzyme inhibitors, protein hydrolysis studies, and research tracking alterations in peptides or proteins in analytical chemistry and pharmaceutical development.