Used as a fluorescent probe in biochemical assays due to its strong emission properties upon binding to specific enzymes or proteins. It is particularly effective in monitoring protease activity, where cleavage of the amide bond leads to a detectable change in fluorescence. This compound also serves as an intermediate in the development of sensors for metal ions in environmental and biological samples. Its high selectivity and sensitivity make it suitable for imaging applications in cellular systems. Additionally, it has been investigated for use in high-throughput screening platforms for drug discovery, especially in targeting disease-related enzymes.