Used as a selective substrate for detecting trypsin and trypsin-like enzymes in biochemical assays. Its structure allows for high specificity in enzymatic cleavage, making it valuable in diagnostic applications and protease activity studies. Commonly employed in colorimetric or fluorometric assays where cleavage releases a detectable signal. Also utilized in research settings to evaluate enzyme kinetics and inhibitor screening in diseases involving abnormal protease activity, such as pancreatitis or certain cancers.