Used extensively in biochemical assays to study protease enzyme activity, particularly for trypsin-like and caspase enzymes. The compound acts as a fluorogenic substrate—when cleaved by the target protease, it releases a fluorescent signal proportional to enzyme activity. This allows real-time monitoring of enzymatic kinetics in cell lysates, purified enzyme preparations, and drug screening platforms. Commonly employed in apoptosis research due to its sensitivity to caspase-3 and related proteases. Also utilized in high-throughput screening for identifying and characterizing protease inhibitors in pharmaceutical development.