Peptidase enzymes are widely used in various industries due to their ability to break down proteins into smaller peptides or amino acids. In the food industry, they are used to improve texture, flavor, and digestibility of products such as cheese, meat, and plant-based proteins. They play a key role in protein hydrolysate production for infant formulas and sports nutrition.

In the pharmaceutical sector, peptidases are involved in drug development, especially in the synthesis and modification of peptide-based therapeutics. They are also used in digestive medications to help treat enzyme deficiencies and support gastrointestinal health.

In biotechnology and research, peptidases are essential tools for protein sequencing, mass spectrometry sample preparation, and recombinant protein purification. They help remove affinity tags from fusion proteins during downstream processing.

The detergent industry incorporates peptidases into laundry and dishwashing formulations to break down protein-based stains like blood, egg, and sweat, enhancing cleaning efficiency at lower temperatures.

Additionally, peptidases are used in leather processing to remove hair and non-fibrous proteins from hides, offering a more eco-friendly alternative to chemical methods.