Alkaline proteinase is widely used in the detergent industry due to its ability to break down protein-based stains such as blood, egg, and grass at high pH levels. It is a key component in laundry and dishwashing detergents, enhancing cleaning efficiency even at low temperatures.

In the leather industry, it aids in the dehairing and bating processes, replacing toxic chemicals and reducing environmental impact. It is also used in the food industry for modifying proteins to improve solubility, emulsification, and nutritional value in products like soy protein and dairy preparations.

In biotechnology and pharmaceuticals, alkaline proteinase is employed for enzyme-based treatments, wound debridement, and in the production of peptides through controlled protein hydrolysis. Its specificity and stability under alkaline conditions make it valuable in industrial processes requiring efficient and selective protein degradation.