α-Chymotrypsinogen A is primarily used in biochemical research as a precursor to the enzyme chymotrypsin. It is often employed in studies focusing on protein folding, enzyme activation, and zymogen processing. Researchers utilize it to understand the mechanisms of protease activation and function, which is crucial in fields like enzymology and molecular biology. Additionally, it serves as a model protein for investigating structural changes during enzymatic activation, aiding in the development of therapeutic strategies targeting protease-related diseases. Its role in these studies contributes to advancements in medical and biotechnological applications.