Fmoc-β-HomoThr(tBu)-OH is widely used in peptide synthesis, particularly in solid-phase peptide synthesis (SPPS). It serves as a building block for the incorporation of β-homothreonine residues into peptide chains. The Fmoc group protects the amino group during synthesis, while the tBu group safeguards the hydroxyl group, ensuring selective reactions. This compound is essential in the development of peptides with modified structures, which are often explored for their potential in drug discovery, biochemical research, and the study of protein interactions. Its application is particularly significant in creating peptides with enhanced stability or specific biological activity.