This compound is primarily used in peptide synthesis as a building block for creating custom peptides. It serves as a protected form of alanine, where the ethyl ester group protects the carboxyl end, and the hydrochloride salt ensures stability. It is particularly useful in solid-phase peptide synthesis (SPPS) for constructing peptide chains with specific sequences. Additionally, it finds application in biochemical research for studying enzyme-substrate interactions and peptide structure-activity relationships. Its stability and ease of incorporation into peptide chains make it a valuable tool in pharmaceutical development and biotechnology.