This chemical is widely used in peptide synthesis, particularly in solid-phase peptide synthesis (SPPS) methods. Its Fmoc (fluorenylmethyloxycarbonyl) protecting group is essential for the stepwise construction of peptides, as it can be selectively removed under mild basic conditions without affecting other functional groups. The 2,4-dimethyl substitution on the phenylalanine side chain enhances the steric hindrance, which can be beneficial in designing peptides with specific structural or functional properties. It is often employed in the development of peptide-based drugs, bioactive peptides, and peptidomimetics, where precise control over peptide sequence and conformation is critical. Additionally, it finds applications in research related to protein engineering and the study of protein-protein interactions.