Fmoc-N-Me-Nle-OH is widely used in peptide synthesis, particularly in solid-phase peptide synthesis (SPPS) methods. It serves as a protected amino acid derivative, where the Fmoc (fluorenylmethyloxycarbonyl) group acts as a temporary protecting group for the amine functionality during the synthesis process. This compound is especially valuable for introducing N-methylated norleucine residues into peptide chains, which can enhance the stability, bioavailability, and biological activity of the resulting peptides. Its application is critical in the development of peptide-based drugs, where precise control over amino acid incorporation is essential. Additionally, it is utilized in research to study peptide structure-activity relationships and to design peptides with specific therapeutic or functional properties.