Fmoc-N-Me-Ala-OH is primarily used in peptide synthesis as a building block. It is particularly valuable in solid-phase peptide synthesis (SPPS) for introducing N-methylated alanine residues into peptide chains. The Fmoc (fluorenylmethyloxycarbonyl) group serves as a protecting group for the amine, allowing selective deprotection during the synthesis process. This compound is essential in creating peptides with specific structural and functional properties, often employed in drug development and biochemical research. Its use enhances peptide stability and modulates biological activity, making it a critical tool in designing therapeutic peptides and studying protein interactions.