N-Carbobenzoxy-DL-norvaline is primarily used in peptide synthesis as a protecting group for amino acids. It helps in preventing unwanted reactions at the amino group during the formation of peptide bonds. This compound is particularly useful in the synthesis of complex peptides and proteins, where selective protection and deprotection of functional groups are crucial. Additionally, it finds application in the preparation of modified peptides for biochemical research and pharmaceutical development. Its role in facilitating controlled and efficient peptide assembly makes it valuable in both academic and industrial settings.