Z-4-fluoro-D-phenylalanine is primarily used in research and development, particularly in the field of biochemistry and molecular biology. It serves as a valuable tool in studying protein synthesis and enzyme mechanisms due to its ability to mimic natural amino acids while introducing a fluorine atom, which can be tracked using techniques like NMR spectroscopy. This compound is also utilized in the development of positron emission tomography (PET) imaging agents, where its fluorine isotope (^18F) helps in visualizing and diagnosing various diseases, including cancer. Additionally, it plays a role in investigating bacterial and fungal growth inhibition, contributing to the development of novel antimicrobial agents. Its unique properties make it a key component in advancing scientific understanding and medical applications.