This compound is primarily used in peptide synthesis as a protecting group for amino acids. The tert-butyloxycarbonyl (OtBu) group helps to shield reactive sites during the synthesis process, ensuring selective reactions and preventing unwanted side reactions. It is particularly useful in solid-phase peptide synthesis (SPPS), where it protects the amino group of sarcosine (Sar), allowing for the stepwise construction of peptides. After the synthesis is complete, the OtBu group can be easily removed under mild acidic conditions, making it a convenient choice for peptide chemists. Its application is crucial in the production of complex peptides and proteins for research, pharmaceuticals, and biotechnology.