This compound is primarily used in peptide synthesis as a protected form of lysine. The benzyloxycarbonyl (Z) group safeguards the amino group, while the tert-butyl ester (OtBu) protects the carboxyl group, allowing selective reactions during peptide chain assembly. It is particularly valuable in solid-phase peptide synthesis (SPPS) and solution-phase synthesis, where protecting groups are essential to prevent unwanted side reactions. Its application is crucial in producing peptides for pharmaceuticals, research, and biotechnology, enabling the creation of complex peptide structures with high precision.