This compound is primarily utilized in peptide synthesis, particularly in solid-phase peptide synthesis (SPPS) methods. It serves as a building block for the introduction of N-methylated threonine residues into peptide chains. The Fmoc (fluorenylmethyloxycarbonyl) protecting group ensures selective deprotection during the synthesis process, allowing for the controlled assembly of complex peptides. Its application is significant in the development of therapeutic peptides, where N-methylation can enhance stability, bioavailability, and resistance to enzymatic degradation. Additionally, it is used in research to study peptide structure-activity relationships and to design peptides with specific biological functions.