Used primarily in biochemical research as a reagent for studying enzyme activities, particularly in the investigation of proline-specific enzymes. It serves as a substrate or inhibitor in enzymatic assays to understand the mechanism and kinetics of these enzymes. Additionally, it is employed in the synthesis of chiral compounds, aiding in the development of pharmaceuticals and fine chemicals. Its role in peptide synthesis and modification is also notable, contributing to advancements in proteomics and drug discovery.