This compound is primarily utilized in the synthesis of peptides and other complex organic molecules. It serves as a protected form of serine, where the tert-butyl group safeguards the carboxyl function and the benzyl group protects the hydroxyl function. This protection is crucial during peptide synthesis to prevent unwanted side reactions. The hydrochloride form enhances the compound's stability and solubility, making it easier to handle in various chemical reactions. Its application is significant in pharmaceutical research, particularly in the development of new drugs and therapeutic agents, where precise control over molecular structure is essential. Additionally, it is used in biochemical studies to explore enzyme mechanisms and protein interactions, providing insights into biological processes and aiding in the design of enzyme inhibitors or activators.