This chemical is primarily used in peptide synthesis, particularly in solid-phase peptide synthesis (SPPS). It serves as a building block for constructing peptides with specific sequences. The Fmoc group protects the amino terminus, allowing for sequential deprotection and coupling of amino acids. The tert-butyl (OtBu) group protects the carboxylic acid side chain of glutamic acid, while the pseudoproline moiety (Psi(Me,Me)Pro) in the threonine residue helps reduce steric hindrance and improve peptide chain assembly efficiency. This compound is valuable in the synthesis of complex peptides, including those used in drug development, biochemical research, and the study of protein-protein interactions. Its design ensures high yield and purity in peptide synthesis processes.