This chemical is primarily used in peptide synthesis, particularly in solid-phase peptide synthesis (SPPS). The Fmoc (9-fluorenylmethoxycarbonyl) group serves as a protecting group for the amine functionality, ensuring selective reactions during the synthesis process. The tert-butyl (tBu) group protects the hydroxyl side chain of threonine, while the Psi(Me, Me)pro moiety introduces a pseudoproline dipeptide structure, which helps to reduce aggregation and improve solubility during peptide chain assembly. This compound is especially valuable in synthesizing complex peptides with challenging sequences, as it enhances efficiency and yield. Its application is critical in producing peptides for pharmaceutical research, drug development, and biochemical studies.