This chemical is primarily used in peptide synthesis, particularly in solid-phase peptide synthesis (SPPS). It serves as a protected dipeptide building block, where the Fmoc group protects the amino terminus, and the tert-butyl group protects the side chain of aspartic acid. The pseudoproline derivative in the threonine residue helps to reduce aggregation and improve solubility during peptide chain assembly, making it valuable for synthesizing complex or long peptides. Its application is critical in the production of therapeutic peptides, peptide-based drugs, and research peptides, where precise control over peptide structure and sequence is essential.