Used in biomedical research to quantify levels of ATG12 protein in cell or tissue lysates. Helps study autophagy, a cellular degradation process linked to cancer, neurodegenerative diseases, and immune responses. Enables researchers to monitor changes in autophagy activity under various experimental conditions such as drug treatment, starvation, or genetic modifications. Commonly used in cell biology, pharmacology, and disease mechanism studies. Provides a reliable and sensitive method for detecting ATG12 conjugation, an essential step in autophagosome formation.