Used in biomedical research to quantify bone sialoprotein (BSP) levels in biological samples such as serum, plasma, and cell culture supernatants. Enables assessment of bone metabolism and mineralization processes. Commonly applied in studies related to osteoporosis, bone regeneration, and periodontal disease. Supports research in evaluating the efficacy of therapeutic agents targeting bone formation or resorption. Also utilized in cancer research, particularly in investigating tumor metastasis to bone, as BSP is associated with aggressive cancer phenotypes. Provides a reliable, sensitive, and specific method for high-throughput screening in clinical and experimental settings.