Used in biomedical research to detect and quantify glycogen phosphorylase isozyme BB levels in rat tissue samples, particularly in studies related to brain injury and neurodegeneration. The kit enables researchers to assess blood-brain barrier integrity and neuronal damage, as GP-BB is released into the bloodstream following cerebral ischemia or traumatic brain injury. It is commonly applied in preclinical studies involving stroke models, neuroprotective drug evaluation, and central nervous system toxicity testing. The ELISA format allows for high sensitivity and specificity in measuring low concentrations of GP-BB in serum or plasma, supporting the development of biomarkers for neurological disorders in experimental animal models.