Used in biomedical research to quantify bone morphogenetic protein 4 (BMP-4) levels in rat bone tissue samples. Helps in studying bone formation, osteoblast differentiation, and skeletal development. Commonly applied in preclinical studies related to bone regeneration, fracture healing, and evaluation of osteoporosis treatments. Also supports research in tissue engineering and stem cell differentiation by measuring BMP-4 expression during cell culture experiments.