Used in research settings to quantify levels of High Mobility Group Box 1 (HMGB-1) protein in biological samples such as serum, plasma, and cell culture supernatants. This kit is particularly valuable in studies related to inflammation, sepsis, autoimmune diseases, and cancer, where HMGB-1 acts as a key mediator in immune responses and cellular damage signaling. By enabling accurate measurement of HMGB-1, it supports investigation into disease mechanisms, therapeutic interventions, and biomarker development. The assay is commonly applied in immunology, oncology, and neuroscience research to assess the role of HMGB-1 in pathological conditions and treatment outcomes.