Used in biomedical research to quantitatively measure levels of human cytochrome c in cell culture supernatants, serum, plasma, and tissue lysates. This assay is particularly valuable in studies related to apoptosis, as cytochrome c is released from mitochondria during the early stages of programmed cell death. The kit enables researchers to assess mitochondrial dysfunction and activation of caspase pathways in conditions such as neurodegenerative diseases, ischemia-reperfusion injury, and cancer therapy response. It is widely applied in drug development and toxicology studies to evaluate compound-induced apoptosis.