Used in biomedical research to quantify human collagen levels in biological samples such as serum, plasma, and tissue extracts. Enables assessment of extracellular matrix remodeling in studies related to fibrosis, wound healing, and connective tissue disorders. Commonly applied in dermatology research to evaluate skin aging and collagen synthesis. Supports investigation of diseases like scleroderma, osteoarthritis, and liver cirrhosis where collagen accumulation or degradation is a key factor. Provides a reliable, high-throughput method for monitoring collagen expression in cell culture and clinical samples.