Used in biomedical research to measure collagen cross-linking levels in rat tissue samples, particularly in studies related to aging, bone health, diabetes, and connective tissue disorders. Helps assess extracellular matrix changes in conditions like osteoporosis, fibrosis, and diabetic complications by quantifying specific collagen cross-links such as pentosidine or other advanced glycation end-products (AGEs). Commonly applied in preclinical studies using rat models to evaluate therapeutic interventions targeting tissue stiffness, wound healing, or metabolic disease effects on structural proteins.