Used in research settings to quantitatively measure levels of ubiquitin activating enzyme (E1) in biological samples such as cell lysates or tissue extracts. This ELISA kit enables scientists to study the regulation of the ubiquitin-proteasome system, which plays a critical role in protein degradation, cell cycle control, DNA repair, and signaling pathways. It is particularly useful in studies related to neurodegenerative diseases, cancer, and autoimmune disorders where dysregulation of ubiquitination is implicated. The assay provides a high-throughput, sensitive, and specific method for monitoring E1 enzyme expression and activity under experimental conditions, supporting both basic research and drug development efforts targeting ubiquitin pathway components.