Used in life science research to detect and quantify recombinant proteins that contain a polyhistidine (HIS) tag. The kit is commonly applied in protein expression studies to confirm successful protein production in cell lysates, purified fractions, or supernatants. It supports high-throughput screening in drug development and is useful in monitoring protein purification steps. Due to its specificity, it minimizes cross-reactivity with non-target proteins, making it reliable for accurate measurement of HIS-tagged proteins in complex biological samples. Widely used in academic, biotech, and pharmaceutical laboratories for consistent and sensitive detection.